List of C. briggsae polymorphisms
(contributed by various labs)
cb-egl-5 Polymorphisms (Scott Baird Lab)
Forward primer: 5'-CAG GGA GCG GAC AAC TTC AAA
GG-3' (exon 1)
Reverse primer: 5'-GGA CAC AGC CCA GGA TTA GCG
AC-3' (exon 2)
Predicted amplification product (AF16 sequence) = 1,723 bp
| Strain |
PCR product |
| cb-egl-5(+) [AF16] |
~1,700 bp |
| cb-egl-5(bdP1) [HK104, HK105, VT847] |
~1,500 bp |
| cb-egl-5(bdP2)
[PB800] |
~1,400 bp |
cb-mes-2 Insertion/Deletion (Scott Baird Lab)
Forward primer: 5'-CAG GCG AAA AAT GCT CTG ACA AGT G
-3'
Reverse primer: 5'-AAT CCG TTC CGT TGA TGG AGG C
-3'
Predicted amplification product (AF16 sequence) = 1,013 bp
| Strain |
PCR product |
| cb-mes-2(+) [AF-16] |
~990 bp |
| cb-mes-2(bdP4) [HK104] |
~880 bp |
cb-mes-4 SNP (Scott Baird Lab)
Forward primer: 5'-CGG AAA TGC GGA AAA TCG
G-3'
Reverse primer: 5'-CTC CTT GAA GAA GAA ATG GTG
GC-3'
Predicted amplification product (AF16 sequence) = 907 bp
| Strain |
Dra I cut |
| cb-mes-4(+) [AF-16] |
None |
| cb-mes-4(bdP3) [HK104] |
680 bp + 227 bp |