C. briggsae Polymorphisms

cb-egl-5 Polymorphisms (Scott Baird Lab)

Forward primer: 5'-CAG GGA GCG GAC AAC TTC AAA GG-3' (exon 1)
Reverse primer: 5'-GGA CAC AGC CCA GGA TTA GCG AC-3' (exon 2)

Predicted amplification product (AF16 sequence) = 1,723 bp

Strain	PCR product
cb-egl-5(+) [AF16]	~1,700 bp
cb-egl-5(bdP1) [HK104, HK105, VT847]	~1,500 bp
cb-egl-5(bdP2) [PB800]	~1,400 bp

cb-mes-2 Insertion/Deletion (Scott Baird Lab)

Forward primer: 5'-CAG GCG AAA AAT GCT CTG ACA AGT G -3'
Reverse primer: 5'-AAT CCG TTC CGT TGA TGG AGG C -3'

Predicted amplification product (AF16 sequence) = 1,013 bp

Strain	PCR product
cb-mes-2(+) [AF-16]	~990 bp
cb-mes-2(bdP4) [HK104]	~880 bp

cb-mes-4 SNP (Scott Baird Lab)

Forward primer: 5'-CGG AAA TGC GGA AAA TCG G-3'
Reverse primer: 5'-CTC CTT GAA GAA GAA ATG GTG GC-3'

Predicted amplification product (AF16 sequence) = 907 bp

Strain	Dra I cut
cb-mes-4(+) [AF-16]	None
cb-mes-4(bdP3) [HK104]	680 bp + 227 bp

Other resources
The Washington University School Of Medicine maintains this website for SNPs identified in C. briggsae genome.