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Integrating genetic and genomic resources for C. briggsae

This was a C. briggsae genetics team presentation at the Caenorhabditis Evolution Worshop in Oeiras, Portugal (May 2006).

Genetic Map Powerpoint (~5 Mb)

Authors: Daniel Koboldt, Bhagwati Gupta, Scott Baird, Helen Chamberlin, and Ray Miller
 
Abstract: Caenorhabditis briggsae is a key emerging model organism. Comparative genomic and genetic studies of C. briggsae with its related model organism C. elegans promise powerful new tools for the identification of eukaryotic gene products and pathways and the study of the conservation/divergence of these. C. elegans and C. briggsae are phenotypically nearly identical but they exhibit significant differences at the genomic sequence level. C. briggsae investigators agree that a key required experimental tool for the organism is a good genetic map tied to the draft sequence. The map is needed to facilitate forward genetics including mapping mutants useful to investigators in the field as a prelude to positional cloning of the mutants. We are constructing a dense genetic map of C. briggsae by genotyping single nucleotide polymorphisms (SNPs) on two sets of recombinant inbred strains. The map will anchor and orient contigs from the draft sequence, making the sequence more useful. We are also assembling collections of mutant animals and are genetically mapping the location of the mutations with high throughput methods using bulked segregants. We hope to use similar techniques to map many mutants found by investigators in the field, and we will make the data and mutants publicly available. The map tools provided by this work will enable a powerful new style of science for comparative genetics and genomics.
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