Daniel Koboldt, Bhagwati Gupta, Scott Baird, Helen Chamberlin, and Ray Miller
Caenorhabditis briggsae is a key emerging model organism. Comparative genomic and genetic
studies of C. briggsae with its related model organism C. elegans promise powerful new tools for the
identification of eukaryotic gene products and pathways and the study of the conservation/divergence of
these. C. elegans and C. briggsae are phenotypically nearly identical but they exhibit significant differences at
the genomic sequence level. C. briggsae investigators agree that a key required experimental tool for the
organism is a good genetic map tied to the draft sequence. The map is needed to facilitate forward genetics
including mapping mutants useful to investigators in the field as a prelude to positional cloning of the
mutants. We are constructing a dense genetic map of C. briggsae by genotyping single nucleotide
polymorphisms (SNPs) on two sets of recombinant inbred strains. The map will anchor and orient contigs
from the draft sequence, making the sequence more useful. We are also assembling collections of mutant
animals and are genetically mapping the location of the mutations with high throughput methods using bulked
segregants. We hope to use similar techniques to map many mutants found by investigators in the field, and
we will make the data and mutants publicly available. The map tools provided by this work will enable a
powerful new style of science for comparative genetics and genomics.
Copyright 2007, Washington University School of Medicine SNP Research Facility. All rights reserved.