Identification of mutated genes in forward genetics screens typically requires positional cloning, in which well-characterized genetic markers are used to
map mutations to a specific region of the genome. However, transposon-mediated insertional mutagenesis offers an alternative for forward genetics screens.
Mos1 is a well-characterized Drosophila transposon that can be utilized to induce mutations in nematodes. Primers specific for the unique sequence tag of Mos1
insertion alleles allow for amplication of the inserted region via PCR. Researchers have already used this method for
rapid identification of mutated genes in C. elegans .
Here we describe Mos1 insertions that were characterized in C. briggsae by
of the genetic map working group.
C. briggsae Mos Insertions
Mos1 insertion in intron of CBG23423
Mos1 insertion in intron of CBG113333 Ras-like protein