A simple method to dramatically increase C. elegans germline microinjection efficiency
In this bioRxiv manuscript, Gibney et al., (2023) describe a paintbrush based method to improve the speed and survival of microinjections in C. elegans. They show that the overall injection efficiency was significantly increased when a paintbrush was used instead of a pick to transfer worms to and from the agarose pad. The technique is simple as demonstrated in their videos.
Since it is harder to generate transgenics in C. briggsae, the Gupta lab will surely be trying this method out!
Use of nematodes to study evolutionary biology
Nematodes have been used as model organisms for evolutionary studies. This article highlights the key findings from research on developmental processes, sex determination, dauer formation, vulva development, male tail formation and developmental regulation of gene expression with regards to evolution. An updated phylogenetic tree of the phylum Nematoda is also provided. This article is an excellent read to summarise evolutionary differences within nematodes for the above mentioned processes.
Haag, E. S., Fitch, D. H. A., & Delattre, M. (2018). From “the Worm” to “the Worms” and Back Again: The Evolutionary Developmental Biology of Nematodes. Genetics, 210(2), 397 LP – 433. https://doi.org/10.1534/genetics.118.300243
C. elegans provide milk to their offsprings through their decomposing bodies
Boroweic et al. (2020) describes the phenomena in which decaying bodies of older C. elegans turn into nutritious fluid for their offsprings using the insulin/IGF-1 insulin pathway.
To read more, please click here
First sibling species of C. elegans
The article by Kanzaki, N. et al. (2018) describes the first sibling species of C. elegans – C. inopinata including its morphological, ecological, behavioural and genetic analysis.
To find out more, please read the article below:
Kanzaki, N., Tsai, I.J., Tanaka, R. et al. Biology and genome of a newly discovered sibling species of Caenorhabditis elegans. Nat Commun 9, 3216 (2018). https://doi.org/10.1038/s41467-018-05712-5
History of the man who revolutionised the field of cell fusion
Benjamin Podbilewicz has spent many years understanding the process of cell fusion and contributing immensely to the filed. To know about his life and his passion for science, click on the link below