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A simple method to dramatically increase C. elegans germline microinjection efficiency

In this bioRxiv manuscript, Gibney et al., (2023) describe a paintbrush based method to improve the speed and survival of microinjections in C. elegans. The authors show a significantly higher injection efficiency when using a paintbrush instead of a pick to transfer worms to and from the agarose pad. The technique is simple as demonstrated in their videos.


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